Sanrokuen Original Data · 2026

First Harvest vs Third Harvest Green Tea: How L-Theanine and Tannins Change

Not all green tea powders have the same chemical profile. Sanrokuen's 2026 laboratory data show clear differences between first-harvest matcha material and third-harvest powdered green tea in L-theanine, free amino acids, tannins, fiber and more.

By Norifumi Kai, SANROKUEN Co., Ltd. · Published August 30, 2026

Many articles talk about matcha or green tea as though they were nutritionally uniform. In practice, harvest season can make a major difference.

The timing of harvest affects leaf maturity, sunlight exposure, chemical composition, taste and the way a tea is best used. A first-harvest, shade-grown tea selected for matcha can be rich in free amino acids and L-theanine. A later-harvest tea may be lower in amino acids but higher in tannin-related values and fiber.

This article explains those differences using Sanrokuen's own 2026 measurement data comparing two real materials:

Comparison chart showing first-harvest matcha and third-harvest powdered green tea measurements
Sanrokuen original measurement data (2026): comparison of first-harvest matcha material and third-harvest powdered green tea.
L-Theanine
3.5% vs 0.4%
First-harvest matcha contained far more theanine than the third-harvest powder.
Tannins
6.2% vs 16.1%
Third-harvest green tea powder showed much higher tannin-related values.
Free Amino Acids
6.0% vs 1.2%
The amino-acid difference between first and third harvest was also very large.

Why harvest season matters in Japanese green tea

Green tea is an agricultural product, not a standardized chemical product. The tea plant changes as the season progresses. Young spring leaves harvested early in the year are different from leaves harvested later in summer.

Several factors help explain why:

Key idea: “High grade” does not mean “highest in every component.” A first-harvest tea may be strongest in theanine and amino acids, while a third-harvest tea may be higher in tannin-related values and fiber.

Sanrokuen's 2026 measurement data

The table below compares the main values measured in the two materials.

Component 2026 First-Harvest Matcha Material
Enju Premium
2026 Third-Harvest Powdered Green Tea
Saemidori + Sayamakori + Yabukita Blend
What stands out?
Total nitrogen 6.9% 4.1% Higher in first-harvest matcha material
Free amino acids 6.0% 1.2% Much higher in first-harvest matcha
Theanine 3.5% 0.4% Very large difference favoring first-harvest matcha
Fiber 18.7% 24.6% Higher in third-harvest powder
Tannins 6.2% 16.1% Much higher in third-harvest powder
Vitamin C 0.43% 0.41% Almost the same

Sanrokuen original measurement data. First-harvest matcha material (Enju Premium): measured May 14, 2026. Third-harvest powdered green tea (blend of Saemidori, Sayamakori and Yabukita): measured August 25, 2026. Measured values vary depending on raw material, cultivar, harvest season and other conditions.

These figures show that the difference between first and third harvest is not minor. It is large enough to change how the tea tastes and how it may be positioned.

How L-theanine changes from first harvest to third harvest

L-theanine is one of the compounds most closely associated with the mellow, soft and umami-rich character of quality Japanese green tea.

In Sanrokuen's data, first-harvest matcha material contained 3.5% theanine, while the third-harvest powdered green tea contained only 0.4%. That is one of the clearest differences in the entire comparison.

The same pattern appears in free amino acids overall: 6.0% in the first-harvest matcha material, compared with 1.2% in the third-harvest powder.

What this suggests

If your interest is in amino-acid-rich tea with a softer, more umami-oriented profile, first-harvest matcha material is far more suitable than later-harvest green tea powder.

This does not prove a specific health effect from one serving. But it does show that if a reader is specifically interested in theanine, it is not enough to ask whether a product is “green tea” or even “powdered green tea.” The harvest season matters.

How tannins change from first harvest to third harvest

The reverse pattern appears in tannins.

Sanrokuen's first-harvest matcha material measured 6.2% tannins, while the third-harvest powdered green tea measured 16.1%. This is a very large increase.

In practical terms, later-harvest tea often shows a stronger, more astringent character. It can also contain a different balance of polyphenol-related compounds compared with first-harvest matcha selected for umami and softness.

To see how harvest season fits together with catechins, cultivar, shading, steaming and brewing temperature, read Why Is Green Tea Astringent?

What this suggests

If a reader is more interested in tannin-rich or catechin-oriented green tea characteristics, a later-harvest green tea powder may be more relevant than high-grade first-harvest matcha.

Other components: fiber, total nitrogen and vitamin C

Fiber

Fiber was 18.7% in the first-harvest matcha material and 24.6% in the third-harvest powder. This higher fiber value in the third-harvest material fits the broader pattern of more mature leaf material.

Total nitrogen

Total nitrogen was 6.9% in the first-harvest matcha material and 4.1% in the third-harvest powder. In tea evaluation, nitrogen-related values often align with richer amino-acid content and a more premium raw-material profile.

Vitamin C

Vitamin C was 0.43% in the first-harvest matcha material and 0.41% in the third-harvest powder. In this comparison, vitamin C was relatively similar and not the main differentiating factor.

Third-harvest powdered green tea from Kumamoto
Third-harvest powdered green tea from Kumamoto. Later-harvest tea can show a different balance of fiber, tannins and taste compared with first-harvest matcha material.

How these differences affect taste and use

First-harvest matcha material

  • Higher free amino acids
  • Much higher theanine
  • Lower tannins
  • Softer taste, more umami, less harsh astringency
  • Better suited to premium matcha-style positioning

Third-harvest powdered green tea

  • Lower amino acids and theanine
  • Higher fiber
  • Much higher tannins
  • Stronger, more astringent profile
  • Potentially more suitable where catechin/tannin orientation matters

These differences help explain why two products can both be called “green tea powder” while having very different taste profiles and very different analytical values.

In other words, it is more useful to ask “What kind of green tea powder is it?” than to assume all powders are nutritionally or sensorially similar.

Are tannins the same as EGCG?

No. This point is important.

Tea tannins are related to the broader group of polyphenols and catechin-related compounds, but tannins are not the same thing as EGCG alone.

EGCG is one specific catechin. Tea also contains other catechins such as EGC, ECG and EC. Therefore, when our data show that third-harvest powder has a higher tannin value, the correct interpretation is:

Accurate wording matters: third-harvest green tea powder appears more tannin-rich, while first-harvest matcha appears more theanine- and amino-acid-rich. That is the strongest conclusion directly supported by the data.

What readers can learn from this comparison

This comparison is useful because it shows that “green tea” is not one single nutritional category.

A person interested in a softer, umami-rich tea and higher theanine values may find first-harvest matcha much more relevant. A person interested in stronger tannin-related values and a more astringent green tea profile may look more closely at later-harvest material.

That distinction can help readers understand not only flavor and price, but also the underlying chemistry of Japanese green tea.

For a broader overview of vitamins, minerals, catechins and caffeine in matcha, see our Matcha Nutrition guide.

Frequently asked questions

Does first-harvest matcha always have more theanine?

Not always in every tea, but in general first-harvest, shade-grown matcha material tends to be richer in theanine and free amino acids than later-harvest tea. Sanrokuen's 2026 data clearly showed this pattern.

Does third-harvest green tea always have more tannins?

Not in every case, but later-harvest tea commonly shows higher tannin-related values and a stronger astringent profile. Our 2026 measurements showed 16.1% tannins in third-harvest powder compared with 6.2% in first-harvest matcha material.

Is third-harvest tea worse than first-harvest tea?

No. It is different. First-harvest tea is often preferred for premium sensory quality and amino-acid richness, while later-harvest tea may be useful when a stronger, more tannin-oriented profile is desired.

Can tannin values be used as EGCG values?

No. Tannins are a broader category related to polyphenols and catechins. EGCG is only one catechin within that broader group.

References

  1. Sanrokuen original measurement data. First-harvest matcha material (Enju Premium), measured on May 14, 2026. Third-harvest powdered green tea (blend of Saemidori, Sayamakori and Yabukita), measured on August 25, 2026.
  2. Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Standard Tables of Food Composition in Japan (Eighth Revised Edition, Supplement 2023).
  3. Kochman J, Jakubczyk K, Antoniewicz J, Mruk H, Janda K. Health Benefits and Chemical Composition of Matcha Green Tea: A Review. Molecules. 2021;26(1):85.

Educational information only. This page discusses measured composition and general tea science for educational purposes. It is not intended to diagnose, treat, cure or prevent disease. Component values vary depending on cultivar, harvest season, growing conditions, processing and storage.